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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Chemotherapy-induced niche perturbs hematopoietic reconstitution in B-cell acute lymphoblastic leukemia

Fig. 1

Chemotherapy treatment reduces the self-renewal property of MSCs. a Construction of B-ALL mouse model. Representative image of GFP positive cells in N-MYC-GFP B-ALL mouse model. b Immunofluorescence imaging of nestin in femoral bone marrow (BM) sections of four groups (Ctrl, Ctrl+A2D, B-ALL and B-ALL+A2D). GFP positive cells represent N-MYC-GFP derived B-ALL cells, DAPI stains cell nucleus. Scale bars represent 100 μm or 50 μm in inset. c Flow cytometric sorting of BM derived MSCs with the markers CD45Ter119CD31CD140a+CD51+ isolated from the BM of the four groups. SSC, side scatter. d Mesensphere-forming assay of the flow-sorted MSCs from the BM of four groups. e Representative brightfield images of secondary CD140a+CD51+ clonal mesenspheres. f Statistical summary of the sphere numbers of four groups in (e). g Statistical summary of the sphere diameter of four groups in (e). All data in this figure were shown as mean ± SD from three separate experiments. * P ≤ 0.05; ** P < 0.01

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