Fig. 2
From: KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc
KPNA2 promoted the glycolytic metabolism in the glioblastoma cells. a Levels of KPNA2 were analyzed by qRT-PCR and immunoblotting in the U87 glioblastoma cells transfected with the lentiviruses expressing small hairpin RNAs of KPNA2 (shKPNA2), wild-type KPNA2 (KPNA2), or a vector with scrambled nonspecific shRNAs(SC). NC is for the original U87 cells that without any handles, GAPDH served as a loading control. b mRNA levels and c enzymatic activities of HK2, PKM2 and PFK1 were determined in the U87 cells transfected with KPNA2-shRNA(siKPNA2), scrambled shRNA(SC) or wild-type KPNA2(KPNA2). Each bar represented the mean ± s.d. from three independent experiments. *P <  0.05, ***P <  0.001. d Lactate production was measured in the indicated cells. Data were presented as the mean ± s.d. from three independent experiments. **P <  0.01. e MRNA levels of MCT1 and MCT4 in the U87 cells. Data were presented as the mean ± s.d. from three independent experiments. *P < 0.05, **P < 0.01. f Relative deoxyglucose uptake was measured in the indicated cells. Each bar represented the mean ± s.d. from three independent experiments. ***P < 0.001. g The expression of GLUT-1 was detected by flow cytometry in the indicated cells, cells were marked with Alexa Flour®647-GLUT1 antibody and the statistic analyze were followed. Data were presented as the mean ± s.d. from three independent experiments. **P < 0.01. ***P < 0.001