Fig. 5
From: KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc
KPNA2 mediated both the translocation and the transcriptional expression of c-myc. The subcellular distribution of c-myc in the KPNA2-shRNA and control transfected cells by nuclear/cytosol fractionation (a) and immunofluorescence (b) was shown. Error bars indicated the mean ± s.d. of three independent views. MEK1/2 was used as the cytoplasmic control and Histone H3 as the nuclear control. *P < 0.05, ***P < 0.001. c Co-immunoprecipitation analysis was presented between KPNA2 and c-myc in the U87 cells. d Expressions of c-myc in the U87 cells knockdown or overexpressed of KPNA2 were determined by immunoblotting. e The U87 and U251 cells were transfected with pGL2-c-myc, a vector encoding KPNA2-shRNA, wild-type KPNA2 or its comparative control, and pSV-Kerilla. Values in graphs represented the mean of Fluc:Rluc activity±s.d. performed in triplicate. **P < 0.01