Fig. 1

Characterization of exosomes derived from B cell lymphoma. a Transmission electron microscopy analysis of exosomes (EXOs) derived from DLBCL cell lines OCI-LY3, SU-DHL-16, and Burkitt’s lymphoma cell line Raji. (scale bar = 100 nm). b Mean diameter and size distribution of B cell-derived EXOs was assessed by Nanoparticle tracking analysis. c Flow cytometry analysis of the tetraspanins CD81 and CD63 on three kinds of lymphoma EXOs. d Western blotting analysis of exosomal biomarkers and cellular proteins in OCI-LY3 EXOs and cell lysates. e B cell-derived EXOs were incubated with 4-μm-diameter aldehyde/sulfate latex beads, and stained with a panel of antibodies, followed by flow cytometry analysis. The EXOs were also stained with isotype-matched irrelevant antibodies respectively, which were used as controls (green line). Representative of the three independent experiments are shown