Fig. 2

Exosomes derived from DLBCL cells harbor tumor associated antigens and internalized by DCs. a Immunoblot analysis of proteins from OCI-LY3 cells and its EXOs for detection of Hsp70, c-Myc, Bcl-2, Mcl-1, xIAP, and Bcl-xL. b DCs were cultured with PKH67 labeled OCI-LY3 EXOs (100 μg/ml) for the indicated times, and the uptake of EXOs was analyzed by immunofluorescence. Insert image represents the results of flow cytometry analysis for detection of PKH67 positive DCs at 24 h. c DCs were cultured with PKH67 labelled OCI-LY3 EXOs for 2 h and 12 h, respectively, and then counterstained with DAPI. Green represents PKH67-labelled EXOs; blue represents nuclei. d Human DC cell line DCS were treated with or without OCI-LY3 EXOs for 24 h, and then the expression of Bcl-2 were analyzed by immunofluorescence method