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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Dual effect of DLBCL-derived EXOs in lymphoma to improve DC vaccine efficacy in vitro while favor tumorgenesis in vivo

Fig. 3

Communication between DLBCL exosomes and recipient cells. a Human DCs were treated with or without OCI-LY3 EXOs (50 μg/mL; DCtex), OCI-LY3 lysates (50 μg/mL; DClys), for 24 h, and stained with anti-CD80, CD83, CD86, and HLA-DR. Flow cytometry analysis was used for the detection of protein expression. b Murine bone marrow stromal cells (BMSC) obtained from C57BL/6 and NOD-SCID mice were incubated with or without OCI-LY3 EXOs (50 μg/mL) for 24 h. Human HLA-DR expression in the BMSC was assessed by flow cytometry analysis. c HUVECs were treated with or without different concentrations of SU-DHL-16 EXOs for 24 h. The expression of B cell markers CD19, CD20, and HLA-DR were analyzed by flow cytometry analysis. d and e PKH-67 labelled-EXOs derived from Raji cells were incubated with Raji cells for the indicated time, and then cells were stained with PE-anti CD19, followed by counterstained with DAPI. EXOs uptake and cellular location was assessed under a fluorescence microscopy

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