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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Dual effect of DLBCL-derived EXOs in lymphoma to improve DC vaccine efficacy in vitro while favor tumorgenesis in vivo

Fig. 4

Effects of exosomes-derived from OCI-LY3 on T cell proliferation and antitumor activity. a Human PBMCs were co-cultured with DCs, OCI-LY3-pulsed DCs (DCtex) and cell lysates of OCI-LY3-pulsed DCs (DClys) for 4 days. Then CD3+, CD4+ and CD8+ T cells were detected by flow cytometric analysis. b T cell proliferation was assessed using a Carboxyfluorescein diacetate succinimidyl ester (CFSE) assay, followed by flow cytometry analysis. c After co-culture for 4 days, concentrations of IL-6, TNF-α, and immunosuppressive cytokines including IL-4 and IL-10 in the supernatants were measured by Cytometric Bead Array Human Th1/Th2 Cytokine Kit (two-tailed t test, *P < 0.05; **P < 0.01; ***P < 0.001). d BALB/C mice were immunized with DCs, A20 EXOs-pulsed DCs, or A20 lysates-pulsed DCs (DClys) for three times intravenous injection at weekly interval before challenged with A20 cells at one week after the last immunization. Then PBMNCs were obtained from the mice, and flow cytometry analysis was performed for the detection of CD3, CD4, and CD8 positive cells. e CD4+ CD25 + FoxP3 + Treg cells in PBMCs from the mice treated with DCtex, DClys, DC or PBS, respectively. (two-tailed t test, *P < 0.05, n = 6). f Spleen lymphocytes and (g) lymph node lymphocytes were harvested from the mice immunized with A20 TEXs, A20 cell lysates or PBS for 7 days, and then were co-cultured with A20 cells at different E: T (effector: target) ratios. Cytolysis efficiency of lymphocytes was evaluated by a lactate dehydrogenase-releasing cytotoxicity assay

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