Fig. 6

Exosomes of B cell lymphoma induce the invasion of human normal cells. a HUVECs, the human skin firoblasts (HSFs) and primary gastric related fibroblasts (SFs) from healthy donors were stimulated with serum-free DMEM medium containing 100 μg/ml OCI-LY3 EXOs, SU-DHL-16 EXOs, and Raji EXOs, respectively. EXOs of HUVECs and PBS were used as controls. Cells were overlaid onto Matrigel-coated Transwell inserts, and then were continue cultured for 24 h. The inserts were fixed in 4% formaldehyde and stained with 0.1% crystal violet. Slides were photographed and the invading cells counted using ImageJ software. b Number of the invading cells in HUVECs group. c Number of the invading cells in HSPs group. d Number of the invading cells in SFs group. Scale bar = 10 μm. Data representative of 3 independent experiments. (two-tailed t test, *P < 0.05; **P < 0.01; ***P < 0.001). e Western blots of MMP2 and MMP9 expression in HUVECs stimulated with 100 μg/ml of lymphoma-derived EXOs for 24 h. f The mRNA expression levels of MMP2 and MMP9 in HUVECs treated with lymphoma EXOs were detected by quantitative real-time PCR. (two-tailed t test, *P < 0.05)