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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Triptolide inhibits Epstein-Barr nuclear antigen 1 expression by increasing sensitivity of mitochondria apoptosis of nasopharyngeal carcinoma cells

Fig. 4

TP reduces the half-life of EBNA1 and induces proteasomal degradation of EBNA1. a HONE1/Akata, HK1/Akata, and C666–1 cells were treated with DMSO (0.01%) or TP (100 nM) for 24 h. Transcriptional levels of EBNA1 were examined by the real-time PCR. b HK1/Akata and (c) HONE1/Akata cells were treated for 48 h with DMSO control (0.01%) or TP (100 nM) in the absence or presence of proteasome inhibitor MG-132 (50 μM) for the last 12 h. d HK1/Akata and (e) HONE1/Akata cells were treated with DMSO control (0.01%) or TP (100 nM) in the absence or presence of proteasome inhibitor MG-132 (50 μM) for 24 h. f CNE1/Akata cells were treated with DMSO control (0.01%) or TP (100 nM) in the absence or presence of the autophagy inhibitor 3-MA (10 mM). g HONE1/Akata and (h) HK1/Akata cells were treated with DMSO (0.01%) or TP (200 nM) in the presence of CHX (25 μg/ml) for indicated times. i HONE1/Akata and (j) HK1/Akata cells were treated with 0.01% DMSO or TP for 6, 12, 24, 48, and 72 h, respectively. Whole-cell extracts were prepared and subjected to Western blot analysis

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