Fig. 5

Over-expression of EBNA1 attenuates mitochondrial apoptosis induced by TP. a HONE1/Akata, b HK1/Akata, c CNE1, and (d) HeLa cells were transfected with pSG5-EBNA1(P-ala) for 4 h, followed by treatment with DMSO control (0.01%) or TP(100 nM) for 44 h, respectively. e HONE1/Akata and (f) Hela cells were transfected with pSG5-EBNA1(P-ala) at indicated concentrations for 4 h, followed by treatment with DMSO control (0.01%) or TP(100 nM) for 44 h, respectively. g CNE1 and (h) CNE/Akata cells were transfected with pGEM-EBNA1(V-val) for 4 h, then treated with DMSO control (0.01%) or TP (100 nM) for 44 h, respectively. i HONE1/Akata and (j) C666–1cells were transfected with EBNA1 siRNA for 4 h, then treated with DMSO control (0.01%) or TP (100 nM), respectively. Whole-cell extracts were prepared and subjected to Western blot analysis