Fig. 6

Over-expression of EBNA1 attenuates TP-induced cell killing and apoptotic effect. a CNE1 and (b) CNE1/Akata cells were transfected with pSG5-EBNA1(P-ala) or pSG5 for 4 h, and treated with TP (100 nM) for 24 h or 48 h. c CNE1 and (d) CNE1/Akata cells were transfected with pGEM-EBNA1(V-val) or pGEM for 4 h, and treated with TP (100 nM) for 24 h or 48 h. Cell viability of NPC cells were detected by a CCK-8 assay. e HONE1/Akata cells were treated with DMSO control (0.01%) or TP (100 nM) for 24 h. After staining with Hoechst 33,258, cells were observed using a fluorescence microscope. f The apoptosis cell numbers were calculated, quantified, and shown by the bar graphs (*: P < 0.05; **: P < 0.01; ***: P < 0.001)