Fig. 2

HJURP promotes HCC cell proliferation both in vivo and in vitro. a The efficiency of HJURP knockdown in HCC-LM3 cells. b Cell growth was detected by CCK-8 assays after 0, 2, 4 and 6 days (n = 8). c Colony formation of HCC-LM3 cells. d Efficiency of HJURP knockdown in Huh7 cells. e Cell growth was detected by CCK-8 assays in Huh7 cells after 0, 2, 4 and 6 days (n = 8). f Colony formation of Huh7 cells. g Efficiency of HJURP overexpression in SMMC-7721 cells. h Cell growth was detected by CCK-8 assays in SMMC-7721 cells. i Colony formation of SMMC-7721 cells. j and k HJURP facilitates the tumorigenicity of HCC cells in vivo. l and m Tumor volumes and weights were measured every 4 days. lenti-HJU#1: HJURP knockdown group#1; lenti-HJU#2: HJURP knockdown group#2; lenti-Ctrl: negative control group; HJURP-OE: HJURP-overexpressing group; and HJURP-Ctrl: control group