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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: HJURP promotes hepatocellular carcinoma proliferation by destabilizing p21 via the MAPK/ERK1/2 and AKT/GSK3β signaling pathways

Fig. 4

a CDK4 overexpression plasmids were transfected into lenti-HJU#1 cells. CDK4 protein expression was detected by western blot. b CCK-8 assay of lenti-HJU#1, lenti-Ctrl and lenti-HJU#1 + CDK4 group. c Colony formation assays of the indicated groups. Approximately 1500 cells/well were seeded and cultured in 6-well plates for 14 days. d Efficiency of p21 knockdown (sip21) in HCC-LM3 cells. e Silencing of p21 in the HJURP-knockdown HCC-LM3 cells rescued cell growth function in CCK-8 assay, f colony formation assay and g cell cycle assay. h p21 overexpression plasmids were transfected into HJURP-overexpressing (HJURP-OE) cells. Efficiency of p21 overexpression was determined by western blot. i p21 overexpression inhibited the colony formation ability of the HJURP-OE SMMC-7721 cells. j Expression of p21 was detected by immunofluorescence in the HCC-LM3 cells. k Nuclear p21 expression was detected in HCC cell lines. CE: cytoplasm. NE: nucleus

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