Fig. 6

HJURP destabilizes p21 in an ERK1/2 and GSK3β-dependent manner. a Protein levels of ERK1/2, p-ERK1/2, GSK3β, p-GSK3β and p21 were detected by western blot in the HJURP knockdown and overexpression cells. b HCC-LM3 cells were incubated with the ERK1/2 pathway inhibitor U0126 (5 μM) and c AKT activator SC-79 (5 μM) for 24 h before lysis for western blot. ERK1/2, p-ERK1/2, GSK3β, p-GSK3β, AKT, p-AKT were detected by western blot. d Immunofluorescence and e western blot were used to measure subcellular distribution of p21. U: U0126; S:SC-79. f and g U0126 and SC-79 reversed the growth inhibition mediated by HJURP-knockdown in HCC-LM3 cells