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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: IL-6-mediated cross-talk between human preadipocytes and ductal carcinoma in situ in breast cancer progression

Fig. 1

Differentiation ability of human preadipocytes was suppressed by co-culture with DCIS.com, and human preadipocytes appear to revert to a fibroblast phenotype. a Experimental scheme illustrating the transwell culture of DCIS.com (DCIS.com) and human preadipocytes (hPreAd). b Proliferation analysis of hPreAd in single or co-culture was assessed by crystal violet assays of six independent experiments. c Analysis of fibroblast-specific protein 1 (FSP1) and alpha-smooth muscle actin (α-SMC) expression in hPreAd in single or co-culture, as assessed by immunostaining. Green: FSP1 or α-SMC-positive. Blue: nucleus. d Analysis of FSP1 and α-SMC expression in hPreAd treated with DCIS.com conditioned medium (CM), as assessed by western blot. e Differentiation analysis of hPreAd in single or co-culture was assessed by Oil Red O staining of six independent experiments. f Analysis of lipoprotein lipase (LPL) and peroxisome proliferator-activated receptor-gamma (PPAR-γ) mRNA expression in hPreAd in single or co-culture, as assessed by RT-PCR and real-time RT-PCR of three independent experiments. All data are presented as the means ± standard errors

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