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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: IL-6-mediated cross-talk between human preadipocytes and ductal carcinoma in situ in breast cancer progression

Fig. 3

IL-6 expression in human preadipocytes was increased by co-culture with human breast DCIS cells. a Experimental scheme illustrating the transwell culture of DCIS.com (DCIS.com) and human preadipocytes (hPreAd). b Measurement of secreted IL-6 expression in single or co-culture, as assessed by ELISA. Data are presented as the means ± standard errors of three independent experiments. c Analysis of IL-6 and IL-6 receptor (IL-6R) mRNA expression levels in DCIS.com and hPreAd in single culture (S) or co-culture (C), as assessed by RT-PCR. d Analysis of IL-6 and IL-6R protein expression levels in DCIS.com and hPreAd in single culture (S) or co-culture (C), as assessed by western blot. e Analysis of IL-6 and IL-6R protein expression levels in DCIS.com and hPreAd treated with each conditioned medium (CM), as assessed by western blot

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