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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells

Fig. 3

Gene expression analysis. Quantitative RT-PCR of Trap (a-c), Ctsk (d-f) and Mmp9 (g-i) in Raw264.7 cells cultured with: (a, d, g) RANKL used as positive control; (b, e, h) conditioned medium harvested from untreated MDA-MB-231cell cultures (CM) or conditioned medium form cells treated with LIPUS (LCM); and (c, f, i) co-cultured with MDA-MB-231 cells untreated (Untreated_CC) or treated (LIPUS_CC) with LIPUS. PCR data were expressed as fold of change (2-ΔΔCt) vs. the calibrator (CTR) (Mean ± SD, n = 3 replicates). Student t test: *p < 0.05, **p < 0.005, ***p < 0.0005. Effect sizes between RANKL and CTR: Trap (a) d = 2.3, p = 0.022; Ctsk (d) d = − 8.0, p = 0.001; and Mmp9 (g) d = − 2.0, p = 0.047

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