Fig. 4

Gene expression in NPC PDX-B with different drug treatments. a RNA expression of nine cell cycle-related genes in NPC PDX-B exposed to five drug treatments based on original normalized (RPKM) RNA seq data (upper), fold change normalization with DMSO control (middle), RT-PCR validation fold change normalized with internal control GAPDH in DMSO (lower). b Western blot of the nine cell cycle-related proteins after DMSO (control), GEM, PAL and GEM+PAL treatment in NPC PDX-B tissues. c Western blot of the nine cell cycle-related proteins in C666–1 cells treated with different concentrations of GEM (0.1, 1, and 10 μM) and PAL (0.1, 1, and 5 μM) after 48 h