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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: SP1-induced lncRNA AGAP2-AS1 expression promotes chemoresistance of breast cancer by epigenetic regulation of MyD88

Fig. 4

AGAP2-AS1 promotes the expression of MyD88 by modifying H3K27 acetylation. a Bioinformatics analysis showed that the promoter of MyD88 had a high enrichment of H3K27ac. b ChIP assay was used to verify the enrichment of H3K27ac in MyD88 promoter in the breast cancer cells and the normal breast epithelial cells. c ChIP assay showed that the enrichment of H3K27ac was higher in the trastuzumab resistant cells than in the parental cells. d MyD88 expression was detected by western blot in breast cancer cells treated with C646 or DMSO. e FISH analysis of the subcellular location of AGAP2-AS1 with a specific probe in breast cancer cells. f ChIP assay showed that AGAP2-AS1 positively regulated the enrichment of H3K27ac at the MyD88 promoter. *p < 0.05, ***p < 0.001

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