Fig. 5

AGAP2-AS1 mediates H3K27 acetylation at the promoter of MyD88 by binding with CBP. a CBP expression was detected by RT-qPCR. b The influence of CBP on MyD88 expression was identified by RT-qPCR (left panel) and western blot (right panel). c ChIP assay by using CBP antibody tested the enrichment at MyD88 promoter. d Western blot analysis indicated that AGAP2-AS1 showed little effect on CBP expression. e RIP experiment showed that anti-CBP antibody could precipitate AGAP2-AS1 in SKBR-3 and BT474 cells. f, g ChIP-qPCR revealed that AGAP2-AS1 influenced the enrichment of CBP and the level of H3K27ac acetylation at the promoter region of MyD88. *p < 0.05, **p < 0.01, ***p < 0.001