Fig. 3

AS-IV inhibited the migration and invasion of tumor cells. Macrophages derived from THP-1 were stimulated with IL-4/IL-13 with or without AS-IV (80 nM) for 48 h, and the conditioned medium (CM) was collected. A549 and H1299 cells were cultured with different CM. (a and b) The effect of AS-IV on A549 and H1299 invasion and migration was evaluated by wound scratch assay and trans-well assay, respectively. (c and d) The mRNA levels of genes associated with migration and angiogenesis in macrophages. Data are presented as the mean ± SEM from three independent experiments. Compared to M0, **p < 0.01, *p < 0.05, and n.s., no significance; compared to M2, ^##p < 0.01, ^#p < 0.05, and n.s., no significance