Fig. 8

AS-IV suppressed M2 macrophage polarization in the subcutaneous mouse model. (a and b) The infiltrated macrophages in tumor tissues were stained with the macrophage marker F4/80 and the M2 marker CD206 by IHC and immunofluorescence, and the quantified data are shown. (c) Western blot analysis of Arg-1 expression in tumor tissues. (d) Macrophages from tumor tissue were stained with antibodies against F4/80 and CD206 and analyzed using flow cytometry. (e) T cells from tissues were stained with antibodies against CD3, CD4, and CD8 and assayed by flow cytometry. Data are presented as the mean ± SEM. N = 6–7 animals per group. Compared to the NS group, **p < 0.01, *p < 0.05, n.s., no significant difference