Fig. 2

MiR-452 directly targets the 3’UTRs of GSK3β. a, predicted miR-30b target sequences in the 3’UTRs of GSK3β. The nucleotide mutants altered in the 3’UTRs of GSK3β are highlighted in light blue. b, real-time quantitative PCR analysis of GSK3β in the indicated cells. c, western blot analysis of GSK3β protein expression in the indicated cells. d, co-transfection of miR-452 mimic/indicated reporter gene in SW480 and HCT116 cells, luciferase activity assay expression. The error bars represent mean ± SD from three independent experiments. e, real-time quantitative PCR analysis of miR-452 and GSK3β expression in 19 human CRC tissues. The adjacent columns in different colors represent the relative expression levels of miR-452 and GSK3β in the same fresh CRC tissue. f, Spearman correlation btween miR-452 and GSK3β (p < 0.001)