Fig. 3

MiR-452 activated Wnt/β-catenin signaling pathway. a, the results of top/fop in indicated cells. b, western blot analysis of proteins produced downstream of Wnt signaling activation in the indicated cells. c, d, real-time quantitative PCR analysis of mRNA expression downstream of Wnt signaling activation in the vector and miR-452 cell lines (c) or in the vector and miR-452-inhibitor cell lines (d). The error bars represent the mean ± SD from three independent experiments. e, results of immunohistochemistry in human CRC tissues. The tumor and normal sections were stained using antibodies against β-catenin, c-myc and cyclin D1