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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: MiR-452 promotes an aggressive colorectal cancer phenotype by regulating a Wnt/β-catenin positive feedback loop

Fig. 4

MiR-452 promoted the proliferation and invasiveness of CRC. a, real-time quantitative PCR analysis was used to measure expression of miR-452 in SW480 and HCT116 CRC cell lines transfected with different amounts of has-miR-452 mimics (10 and 20 nM). b, typical images from the colony formation assay; every well contained the number of cells> 50 cells. The colony count was of an entire well and the error bars represent mean ± SD from three independent experiments. c, d, the MTT assay showed divergent cell growth between SW480-Vector and SW480-mimic (c), or between HCT116-vector and HCT116-mimic (d). e, more cells transfected across the Matrigel-precoated membrane in cell lines treated with miR-452 mimics. f, g, h, representative results of the wound-healing assay in a fixed location at 4 regular intervals. i, tumor xenograft model. SW480-vector and SW480-miR-452 mimic cells were injected into the hindlimbs of nude mice (n = 6). Data points are presented as the mean tumor volume ± SD

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