Fig. 5

Inhibition of miR-452 reduced the proliferation and invasiveness of CRC. a, real-time quantitative PCR analysis was used to measure expression of miR-452 in SW620 and HCT15 CRC cell lines transfected with different amounts has-miR-452 inhibitor (10 and 20 nM). b, typical images from the colony formation assay; every well contained > 50 cells. The colony count was of an entire well and the error bars represent mean ± SD from three independent experiments. c, d, the MTT assay showed divergent cell growth between SW620-NC and SW620-inhibitor (c), or between HCT15-NC and HCT15-inhibitor (d). e, fewer cells transfected across the Matrigel-precoated membrane in cell lines treated with a miR-452 inhibitor. f, g, h, representative results of the wound-healing assay in a fixed location at 4 regular intervals. i, tumor xenograft model. SW620-NC and SW620-miR-452 inhibitor cells were injected into the hind limbs of nude mice (n = 6). Data points are presented as the mean tumor volume ± SD