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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: MiR-452 promotes an aggressive colorectal cancer phenotype by regulating a Wnt/β-catenin positive feedback loop

Fig. 7

The promoter of miR-452 binds transcription factor LEF1. a, b, c, d, the results of top/fop and miR-452 expression in the indicated cells. The Wnt pathway was activated in cell lines treated with Wnt3a (a), and expression of miR-452 was upregulated in the same cell lines (c). The Wnt pathway was inhibited in cell lines treated with KYA1797K (b), and expression of miR-452 was downregulated in the same cell lines (d). e, the promoter of miR-452 contained 6 segments. f, luciferase assay was used to confirm the activity of the miR-452 promoter. g, h, the second segment of the miR-452 promoter binds to the LEF1 transcription factor based on PCR (g) and real-time PCR (h). i, MiR-452 promoted growth and invasion of colorectal cancer cell by regulating GSK3β-TCF4/LEF1 positive feedback loops

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