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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: HSP90AA1-mediated autophagy promotes drug resistance in osteosarcoma

Fig. 3

Autophagy mediates HSP90AA1-induced sensitivity of osteosarcoma cells to chemotherapy. a and b HSP90AA1-overexpressing MG-63 cells were treated with Cis (20 μmol/L) or Dox (0.2 μg/mL) for 24 h. The protein levels of LC3, p62 and both cleaved and total PARP were assayed by Western blot (a). Autophagic flux were analyzed by mRFP-GFP-LC3 construct (b). c HSP90AA1-overexpressing MG-63 cells were treated with 3-methyladenine (3-MA, 5 mM). Autophagy activity indicated by LC3 levels was analyzed by western blot. d and e HSP90AA1-overexpressing MG-63 cells were treated with Dox (0.2 μg/mL) for 24 h with or without 3-MA (5 mM 2 h). Apoptosis was assessed by analysis of Annexin V-PE/PI staining (d, n = 3; *, p < 0.05 versus pHBLV control group) and proliferation was analyzed by CCK-8 assay (e, n = 3; *, p < 0.05 versus pHBLV control group). NS, not significant

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