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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: HSP90AA1-mediated autophagy promotes drug resistance in osteosarcoma

Fig. 5

HSP90AA1 promotes autophagy and inhibits apoptosis through PI3K/Akt/mTOR pathway and JNK/P38 pathway respectively. a and b HSP90AA1-overexpressing MG-63 cells and HSP90AA1 shRNA transfected MG-63 cells were treated with Dox (0.2 μg/mL) for 24 h. Total cell lysates were subjected to western blot analysis of key proteins in the PI3K/Akt/mTOR pathway. c Control shRNA/HSP90AA1 shRNA transfected MG-63 cells were treated with Dox (0.2 μg/mL) for 24 h with or without LY294002 (10 μM). Then autophagy was analyzed by measuring LC3 puncta formation (n = 3; *, p < 0.05 versus control shRNA group). NS, not significant. d MG-63 cells transfected with control/HSP90AA1 shRNA for 48 h and were treated with Dox (0.2 μg/mL) for 24 h. Total cell lysates were subjected to western blot analysis of key proteins in JNK/p38 pathway

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