Fig. 5

TIPE3 suppresses NPC cell progression in vitro and in vivo. a TIPE3 expression in CNE2 and SUNE1 cells stably overexpressing HA-tagged-TIPE3 or transfected with the empty vector; b-e Representative results of cell viability (b), colony formation (c), migration (d), and invasion (e) abilities of CNE2 and SUNE1 cells after stably overexpressing TIPE3 or vector; f-h SUNE1 cells stably overexpressing TIPE3 or vector were subcutaneously injected into the right and left dorsal flank of mice (n = 5); The tumor nodules (f), volumes (g), and weights (h) in mice xenografts; (i) SUNE1 cells stably overexpressing TIPE3 or vector was injected into the tail veins of mice (n = 5 in each group); Representative images and quantification of macroscopic tumor nodes on the surface of lung tissues and in lungs stained with H&E. Scale bar, 100 mm; Mean ± SD; *, P < 0.01