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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2)

Fig. 5

FGF9 and CCND2 were two direct target genes of miR-4317. a, b TGFBR1 and CCNE2 were identified as potential regulatory targets of miR-4317 by considering the downregulation of genes using prediction tools and qRT-PCR. c, d The expression levels of FGF9 and CCND2 mRNA and protein were measured by qRT-PCR and western blot analysis using GAPDH as the loading control after transfection of miR-4317 mimic in the A973 and A549 cell lines, respectively. e, f The expression levels of FGF9 and CCND2 mRNA and protein were measured by qRT-PCR and western blot analysis using GAPDH as the loading control after transfection of miR-4317 inhibitors in the GLC82 and H157 cell lines, respectively. g, h Dual-luciferase reporter assay. The relative luciferase activity was normalized to the Renilla luciferase activity assay after co-transfection of cells with miR-4317 mimic and pmiR-RB-REPORT™ constructs containing WT or MUT FGF9 and the CCND2 3’-UTR region in A973 and A549 cell lines. Data are presented as the mean values ± SD from triplicate experiments. ♦p < 0.05

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