Fig. 1From: BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancerGdf2 deletion increases tumor growth in the E0771 mammary cancer model. E0771 cells were injected in the 4th mammary gland and tumor growth was assessed by caliper measurement every 2 to 3 days after tumor detection (a) and tumor weight measured (b) at the end of the experiment, 9 days after tumor detection (WT n = 10, Gdf2−/− n = 14, 1 representative experiment out of 4). c Tumor necrosis area quantification (% of total tumor area) (WT n = 8, Gdf2−/− n = 14, 2 experiments). d-i Representative images and quantitative analysis of the tumors stained for PCNA (d, e), cleaved-caspase 3 (black arrowheads f, g), (WT n = 11, Gdf2−/− n = 18, 2 experiments). Scale bar 50 μm), and TUNEL (h, i) (WT n = 11, Gdf2−/− n = 15, 1 experiment. Scale bar 100 μm) (a) Data are the mean ± SEM. Statistical analysis: Two-way matched ANOVA. (b, c, e, g, i) Data are the median ± interquartile range. Statistical analysis: Mann-Whitney test. *p ≤ 0.05 and **p ≤ 0.01 significantly differentBack to article page