Fig. 1

piR-DQ590027 increases the permeability of glioma-conditioned normal BBB and inhibits the expression of tight junction-related proteins. (a) Relative expression of piR-DQ590027 in ECs and GECs on quantitative real-time PCR. Data represent the mean ± SD (n = 3, each). **P < 0.01 vs. the EC group. (b) Transfection efficiency of piR-DQ590027 in GECs. (c) Effects of piR-DQ590027 overexpression and knockdown on TEER values in the in vitro glioma-conditioned normal BBB model. (d) Effects of piR-DQ590027 overexpression and knockdown on HRP flux in the in vitro glioma-conditioned normal BBB model. (e) Effects of piR-DQ590027 overexpression and knockdown on the expression of tight junction-related proteins on western blot. Data of B-E represent the mean ± SD (n = 3, each). *P < 0.05 vs. the piR-DQ590027 (+) NC group, **P < 0.01 vs. the piR-DQ590027 (+) NC group, ^#P < 0.05 vs. the piR-DQ590027 (+) NC group, ^##P < 0.01 vs. the piR-DQ590027 (+) NC group. (F) Effects of piR-DQ590027 overexpression and knockdown on the expressions and distributions of tight junction-related proteins on immunofluorescence (n = 3, each). Scale bar, 30 μm