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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB

Fig. 4

miR-153 and miR-377 overexpression increases the permeability of glioma-conditioned normal BBB and reduces tight junction-related protein expression. (a) miR-153 and miR-377 expression levels in ECs and GECs on quantitative real-time PCR. Data represent the mean ± SD (n = 3, each). *P < 0.05 vs. the EC group. (b) Transfection efficiency of miR-153 and miR-377 in GECs. (c) Effects of miR-153 and miR-377 on TEER values in the in vitro glioma-conditioned normal BBB model. (d) Effects of miR-153 and miR-377 on HRP flux in the in vitro glioma-conditioned normal BBB model. (e) Effects of miR-153 on the expression of tight junction-related proteins on western blot. (f) Effects of miR-153 on the expression of tight junction-related proteins on western blot. Data of b-f represent the mean ± SD (n = 3, each). *P < 0.05 vs. the pre-NC group, **P < 0.01 vs. the pre-NC group, #P < 0.05 vs. the anti-NC group, ##P < 0.01 vs. the anti-NC group. (g) Effects of miR-153 on the expression of tight junction-related proteins and their distributions on immunofluorescence. Scale bar, 30 μm. (h) Effects of miR-377 on the expression of tight junction-related proteins and their distributions on immunofluorescence. Scale bar, 30 μm

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