Fig. 6

FOXR2 knockdown increases the permeability of glioma-conditioned normal BBB by decreasing the transcription of tight junction-related proteins. (a) FOXR2 expression levels in ECs and GECs on quantitative real-time PCR and western blot assay. Data represent the mean ± SD (n = 3, each). *P < 0.05 vs. EC groups. (b) Transfection efficiency of FOXR2 overexpression and knockdown on quantitative real-time PCR and western blot assay. (c) Effects of FOXR2 on TEER values and HRP flux in the in vitro glioma-conditioned normal BBB model. (d) Effects of FOXR2 on the mRNA and protein levels of tight junction-related proteins on quantitative real-time PCR and western blot assay. Data of b-d represent the mean ± SD (n = 3, each). *P < 0.05 vs. the FOXR2 (+) NC group, ^#P < 0.05 vs. the FOXR2 (-) NC group; ^##P < 0.05 vs. the FOXR2 (-) NC group. (e) Effects of FOXR2 on the expression of tight junction-related proteins and their distribution on immunofluorescence (n = 3, each). Scale bar, 30 μm. (f) FOXR2 bound to the promoter of ZO-1, occludin, and claudin-5 in GECs