Fig. 7

FOXR2 mediates the effects of miR-153 (miR-377) on the permeability of glioma-conditioned normal BBB. (a) Effects of miR-153 and miR-377 on FOXR2 expression. Data represent the mean ± SD (n = 3, each). **P < 0.01 vs. the pre-NC group, ^#P < 0.05 vs. the anti-NC group. (b) Relative luciferase activity in HEK293T cells co-transfected with pmirGLO-FOXR2-Wt (pmirGLO-FOXR2-Mut) and agomir-377 (agomir-153). Data represent the mean ± SD (n = 3, each). *P < 0.05 vs. the FOXR2-3’-UTR-Wt + miR-377 (+) miR-153 (+) group. (c) TEER value was measured to evaluate the effects of FOXR2 and miR-153 (miR-377) on glioma-conditioned normal BBB integrity. (d) HRP flux was measured to evaluate the effects of FOXR2 and miR-153 (miR-377) on the permeability of glioma-conditioned normal BBB. (e) Western blot was performed to evaluate the effects of FOXR2 and miR-153 on tight junction-related proteins. (f) Western blot was performed to evaluate the effects of FOXR2 and miR-377 on tight junction-related proteins. Data of C-F represent the mean ± SD (n = 3, each). *P < 0.05 vs. the pre-NC + FOXR2 (+) NC group, ^#P < 0.05 vs. the pre-miR-153 (pre-miR-377) + FOXR2 (+) NC group