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Table 3 Primers used for ChIP experiments

From: Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB

Gene

Binding site or Control

Sequence (5′- > 3′)

Product size (bp)

Annealing temperature (°C)

ZO-1

PCR1

F:CCGGCAGGTTTGCGTG

102

58

R:GGCTTCGCCACGTAACTTC

  

PCR2

F:AACGAGAGCAACGCTTCTGA

166

55

R:GCCGATACCCGACAGTTGTT

  

PCR3

F:CCGCAGACACCAAAAATCCC

210

54

R:ACGCATGTCATCATAGCTCCT

  

occludin

PCR1

F: GAATTGGTCACCGAGGGAGG

107

59

R:GACCTGGCTCTCGGTTCCT

  

PCR2

F: AAGGTTCCATCCGAAGCAGG

203

56

R:CTGAAACTCGGATGGGAGGG

  

PCR3

F:GAGTTTTGTTCACTCGTGTGTGT

105

56

R:TGTTGTGTTGGGTAGAAGGC

  

claudin-5

PCR1

F: AGAAGGCAGTGGTCCTCAGA

185

62

R: CTTCTCCCCCTTCAGATGGC

  

PCR2

F:CGAAGTAGGTGAGCAGACCC

204

56

R:CACTATGTTGGTCGAGGCCC

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