From: Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
Gene | Binding site or Control | Sequence (5′- > 3′) | Product size (bp) | Annealing temperature (°C) |
---|---|---|---|---|
ZO-1 | PCR1 | F:CCGGCAGGTTTGCGTG | 102 | 58 |
R:GGCTTCGCCACGTAACTTC | Â | Â | ||
PCR2 | F:AACGAGAGCAACGCTTCTGA | 166 | 55 | |
R:GCCGATACCCGACAGTTGTT | Â | Â | ||
PCR3 | F:CCGCAGACACCAAAAATCCC | 210 | 54 | |
R:ACGCATGTCATCATAGCTCCT | Â | Â | ||
occludin | PCR1 | F: GAATTGGTCACCGAGGGAGG | 107 | 59 |
R:GACCTGGCTCTCGGTTCCT | Â | Â | ||
PCR2 | F: AAGGTTCCATCCGAAGCAGG | 203 | 56 | |
R:CTGAAACTCGGATGGGAGGG | Â | Â | ||
PCR3 | F:GAGTTTTGTTCACTCGTGTGTGT | 105 | 56 | |
R:TGTTGTGTTGGGTAGAAGGC | Â | Â | ||
claudin-5 | PCR1 | F: AGAAGGCAGTGGTCCTCAGA | 185 | 62 |
R: CTTCTCCCCCTTCAGATGGC | Â | Â | ||
PCR2 | F:CGAAGTAGGTGAGCAGACCC | 204 | 56 | |
R:CACTATGTTGGTCGAGGCCC | Â | Â |