Fig. 1

FBP1 is responsible for modulating the BET inhibitor sensitivity in PDAC. a, PANC-1 cells were infected with lentivirus expressing control, FBP1-specific shRNAs. After 48 h infection, shControl cells were transfected with pcDNA3.1 or Flag-FBP1 constructs. All cells were treated with different doses of indicated chemicals 24 h post-transfection. The cell viability was measured by MTS assay. Heat map showing the IC50 ratio (log₂ (IC50 ratio)) between shControl versus shControl, knockdown FBP1 versus shcontrol or overexpression FBP1 versus control treated with indicated chemicals. b, PANC-1 cells were infected with lentivirus expressing control, FBP1-specific shRNAs. After 48 h infection, shControl cells were transfected with pcDNA3.1 or Flag-FBP1 constructs. Cells were treated with different doses of JQ1 24 h post-transfection. The cell viability was measured by MTS assay. Data shown are mean values ± SD from six replicates. c-f, PANC-1 cells were infected with lentivirus expressing control or FBP1-specific shRNAs. After 72 h infection, cells were harvested for MTS assay (c), western blotting (d), caspase 3 activity assay (e) and colony formation assay (f). All data are shown as mean values ± SD (n = 3). n.s., not significant, ** p < 0.01 comparing to the shControl group