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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: FBP1 loss contributes to BET inhibitors resistance by undermining c-Myc expression in pancreatic ductal adenocarcinoma

Fig. 1

FBP1 is responsible for modulating the BET inhibitor sensitivity in PDAC. a, PANC-1 cells were infected with lentivirus expressing control, FBP1-specific shRNAs. After 48 h infection, shControl cells were transfected with pcDNA3.1 or Flag-FBP1 constructs. All cells were treated with different doses of indicated chemicals 24 h post-transfection. The cell viability was measured by MTS assay. Heat map showing the IC50 ratio (log2 (IC50 ratio)) between shControl versus shControl, knockdown FBP1 versus shcontrol or overexpression FBP1 versus control treated with indicated chemicals. b, PANC-1 cells were infected with lentivirus expressing control, FBP1-specific shRNAs. After 48 h infection, shControl cells were transfected with pcDNA3.1 or Flag-FBP1 constructs. Cells were treated with different doses of JQ1 24 h post-transfection. The cell viability was measured by MTS assay. Data shown are mean values ± SD from six replicates. c-f, PANC-1 cells were infected with lentivirus expressing control or FBP1-specific shRNAs. After 72 h infection, cells were harvested for MTS assay (c), western blotting (d), caspase 3 activity assay (e) and colony formation assay (f). All data are shown as mean values ± SD (n = 3). n.s., not significant, ** p < 0.01 comparing to the shControl group

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