Skip to main content

Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: FBP1 loss contributes to BET inhibitors resistance by undermining c-Myc expression in pancreatic ductal adenocarcinoma

Fig. 4

FBP1 negatively regulates c-Myc in PDAC. a and b, PANC-1 and SW1990 cells were infected with lentivirus expressing control, FBP1-specific shRNAs. 72 h after infection, cells were harvested for western blot analysis (a) and RT-qPCR (b). Data are shown as means ± SD (n = 3). n.s., not significant. c-d, PANC-1 cells cells were infected with lentivirus expressing control, FBP1-specific shRNAs. 48 h after infection, cells were transfected with indicated plasmids. 24 h post transfection, cells were harvested for western blot analysis (c) and RT-qPCR (d). Data are shown as means ± SD (n = 3). n.s., not significant. e, expression of c-Myc and FBP1, as determined by Western blot, in eight paired primary pancreatic cancer tissues (T) and the matched adjacent non-tumor tissues (N) from the same patient. β -Tubulin served as a loading control. f, c-Myc and FBP1 proteins were quantified and normalized to the quantified value of β-Tubulin. The quantified value of c-Myc and FBP1 in cancer tissues versus normal tissues was shown in Log2 (T/N). g, The correlation analysis of mRNA expression levels between FBP1 and Myc was analyzed from TCGA dataset including 178 pancreatic ductal adenocarcinoma. p = 0.784, R = 0.0207. h, images of IHC analysis of FBP1 and c-Myc protein expression on TMA (n = 50) tissue sections. Scale bars are shown as indicated. i, correlation analysis of the immunoreactivity score of expression levels of FBP1 and c-Myc proteins in human PDAC specimens (n = 50). Pearson’s product-moment correlation co-efficiency and the p values are also shown

Back to article page