Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: FBP1 loss contributes to BET inhibitors resistance by undermining c-Myc expression in pancreatic ductal adenocarcinoma

Fig. 5

FBP1 promotes c-Myc degradation in pancreatic cancer cells. a and b, western blot analysis of whole cell lysate of PANC-1 cells transfected with the indicated constructs. Cells were treated with or without 20 μM of MG132 for 8 h before harvest (a). The c-Myc protein was quantified and normalized to the quantified value of β-Tubulin (b). c and d, PANC-1 cells were infected with indicated constructs. After 72 h, cells were treated with 50 μg/μl cycloheximide (CHX). At different time points, cells were harvested for western blot analysis. At each time point, the intensity of FBP1 was normalized to the intensity of β-Tubulin (loading control) first and then to the value at the 0-h time point. e, PANC-1 and SW1990 cells infected with indicated constructs. Western blot analysis of whole cell lysate of PANC-1 and SW1990 cells after 72 h infection. Cells were treated with or without 20 μM of MG132 for 8 h before harvest. f-i, PANC-1 cells were transfected with indicated constructs. 24 h post transfection, cells were harvested for western blot analysis (f and g), RT-qPCR (h) and glucose consumption measurement (i). Data are shown as means ± SD (n = 3). n.s., not significant; ***, P < 0.001. j, western blot analysis of whole cell lysate of PANC-1 cells transfected with indicated constructs. Cells were treated with or without 20 μM of MG132 for 8 h before harvest. k, western blot analysis of whole cell lysate of PANC-1 cells transfected with indicated constructs. l, western blot analysis of whole cell lysate of PANC-1 cells transfected with indicated constructs. m, western blot analysis of whole cell lysate of PANC-1 cells transfected with indicated constructs. Cells were treated with or without 20 μM of MG132 for 8 h before harvest

Back to article page