Fig. 3

PHF8 regulates the expression of EMT markers. a Western-blot analysis of expression of SNAI1, VIM/ Vimentin, CDH2/ N-cadherin and E-cadherin. b, d quantification of E-cadherin protein amount. c, e qRT-PCR analysis of E-cadherin mRNA expression in SMMC-7721 and Huh7 cells with PHF8 knockdown and HepG2 and SK-Hep-1 cells with PHF8 exogenous overexpression. f-h Analysis of E-cadherin protein and mRNA expression after SNAI1 knockdown by siRNA in HepG2 and SK-Hep-1 cells with PHF8 overexpression. siCtrl was used for negative control. Data were presented by mean ± SD from three independent experiments