Fig. 5

FIP200 of exogenous overexpression reverses the effect of PHF8-silencing on autophagy. a qRT-PCR and western-blot analysis of FIP200 expression in SMMC-7721 and Huh7 cells with exogenous overexpression of FIP200. Vector represented empty plasmid for negative control. b Representative immunoblot result of LC3B and p62 in SMMC-7721 and Huh7 cells with co-transfection of indicated shRNAs and plasmids (Vector or HA-FIP200) after cultured in complete medium with 10% FBS or EBSS starvation condition with or without CQ (100 μmol) for 8-h. c Representative fluorescence images of autophagosomes and autolysosomes in SMMC-7721 and Huh7 cells with co-transfection of shRNAs (shCtrl or shPHF8) and plasmids (Vector or HA-FIP200) (magnification, × 400). d Quantification of autophagosomes and autolysosomes from random 5 high-power fields of the merged images of each group. * P < 0.05, ** P < 0.01. Data were presented by mean ± SD from three independent experiments