Fig. 1

Relative SPAG5 expression in HCC tissues and HCC cells. a, Relative mRNA levels of SPAG5 in HCC tissues and in paired non-tumor tissues. SPAG5 expression was examined by qPCR and normalized to GAPDH expression. Statistical differences between samples were analyzed with paired samples t-test (n = 136, ^**p < 0.01). b and c, Determination and quantification of SPAG5 protein levels in HCC tissues (n = 60) and in paired non-tumor tissues (n = 60) by western blotting. Tubulin was used as a loading control. d, Representative images (left) and quantification (right) of SPAG5 staining in 136 paired HCC tissues. Scale bar, 50 μm. e, qRT-PCR analysis of SPAG5 mRNA levels in HCC cell lines (Huh7, HCCLM3, SMCC7721 and Hep3B) and normal cells (HL-7702) ^*p < 0.05. f, Quantification of SPAG5 protein expression using western blot analyses in HCC cell lines and HL-7702 cell