Fig. 5

SCARA5 is key for SPAG5-mediated HCC proliferation. a, The knockdown of SCARA5 expression markedly inhibited the increase in SCARA5 expression observed in Huh7/shSPAG5 cells. b and c, CCK-8 assay and Flow Cytometry assay showed that SCARA5 inhibition rescued the decreased cell proliferation ability of Huh7/shSPAG5 cells. ^*p < 0.05. d, results are expressed as calculated percentage of Annexin-V-positive cell population. ^*p < 0.05. e. Western blotting was used to detect the expression of SPAG5 and SCARA5. The overexpression of ectopic SCARA5 attenuated the loss of SCARA5 expression in Hep3B/p-SPAG5 cells. f and g, CCK-8 assay and Flow Cytometry assay showed that the overexpression of ectopic SCARA5 significantly reduced the SPAG5-enhanced cell proliferation observed in Hep3B/p-SPAG5 cells. ^*p < 0.05. h, results are expressed as calculated percentage of Annexin-V-positive cell population. ^*p < 0.05. i–l, The tumor sizes and tumor weight were measured in each groups. ^*p < 0.05