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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Yes-associated protein (YAP) binds to HIF-1α and sustains HIF-1α protein stability to promote hepatocellular carcinoma cell glycolysis under hypoxic stress

Fig. 3

Hypoxia activated YAP and induced YAP nuclear translocation in HCC cells. a, b Western blot showed the expression of HIF-1α, LATS1, p-LATS1(S909), YAP and p-YAP(S127) in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. c Immunofluorescence showed the location of YAP in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. d Western blot showed the expression of YAP in the nuclear fraction or cytoplasm in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. e Real-time PCR showed the mRNA expression of YAP-regulated genes (CTGF, CYR61, AREG and EDN1) in HCC cells under normoxia or hypoxia (1%O2) for 24 h. Data are shown as the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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