Fig. 4
Hypoxia promoted HCC cell glycolysis in vitro. a Relative glucose uptake in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. b Relative lactate production in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. c, d The overall glycolytic flux of HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) were analyzed by ECAR using seahorse instrument. 2-DG, 2-deoxyglucose. e Real-time PCR analysis for the expression of Glut1, PKM2, LDHA and PGK1 genes in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h, respectively. f Western blot showed the expression of Glut1, PKM2, LDHA and PGK1 protein in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h, respectively. g, h Migration and invasion assays in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. (magnification: × 100). i, j The expression of MMP2 and MMP9 protein in HepG2 and Huh7 cells under normoxia (20%O2) or hypoxia (1%O2) for 24 h. Data are shown as the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01