Fig. 5

Silencing YAP inhibited HCC cell glycolysis under hypoxia. a, b Real-time PCR and western blot were used to examine knockdown efficiency of YAP in HepG2 and Huh7 cells transfected with YAP siRNA. c, d Analysis of the uptake of glucose and production of lactate in HepG2 cells and Huh7 cells under hypoxia (1%O₂) for 24 h after transfected with YAP siRNA. e, f The overall glycolytic flux of HepG2 and Huh7 cells under hypoxia (1%O₂) after transfected with YAP siRNA were analyzed by ECAR using seahorse instrument. 2-DG, 2-deoxyglucose. g, h Western blot showed the expressions of HIF-1α and PKM2 in HCC cells under hypoxia (1%O₂) for 24 h after transfected with YAP siRNA. i, j Migration and invasion assays in HepG2 and Huh7 cells under hypoxia (1%O₂) for 24 h after transfected with YAP siRNA. (magnification: × 100). k, l The expression of MMP2 and MMP9 protein in HepG2 and Huh7 cells under hypoxia (1%O₂) for 24 h after transfected with YAP siRNA. Data are shown as the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001