Fig. 2

SLCO4A1-AS1 knockdown inhibits cell proliferation, migration and invasion in CRC. a Stable knockdown of SLCO4A1-AS1 was checked by qRT-PCR in HCT116 and SW480 cells transfected with lentivirus containing SLCO4A1-AS1 shRNA sequence. siNC: shRNA negative control; siLINC: shSLCO4A1-AS1. b, c The effect of SLCO4A1-AS1 expression on cell proliferation was evaluated with HCT116 and SW480 cells by CCK-8 assays and colony formation assays. d Cell cycle distribution was determined by FACS. e, f The effect of SLCO4A1-AS1 knockdown on the migration and invasion of CRC cells was assessed by a Transwell and Matrigel assay, respectively. Results are representative of the average counts from five random microscopic fields. Scale bar, 50 μm. g SLCO4A1-AS1 knockdown increased the expression of epithelial markers of α-catenin and E-cadherin while downregulated that of mesenchymal markers of Fibronectin and Vimentin in HCT116 and SW480 cells. h Knockdown of SLCO4A1-AS1 promoted the apoptosis of HCT116 and SW480 cells. Cells were stained with Annexin V/PI and analyzed by FACS. *p < 0.05, **p < 0.01 and ***p < 0.01