Fig. 3
SLCO4A1-AS1 activates Wnt/β-catenin signaling in CRC. a Gene Set Enrichment Analysis (GSEA) showed that SLCO4A1-AS1 highly expression was positively related to cell division and G1/G2 phase transition. Samples in Li’s cohort (GSE104836) were divided into 2 groups based on expression of SLCO4A1-AS1. NES, normalized enrichment score. b GSEA showed that SLCO4A1-AS1 expression was positively correlated with canonical Wnt signaling according to Li’s cohort (GSE104836). c Expression heatmap of the target genes of Wnt/β-catenin signaling showed that more activation of Wnt/β-catenin signaling appeared in SLCO4A1-AS1high group. d, e Real-time PCR confirmed that SLCO4A1-AS1 knockdown significantly downregulated Wnt/β-catenin signaling in HCT116 and SW480 cells. f The protein levels of AXIN2, LGR5 and MYC in HCT116 and SW480 cells after SLCO4A1-AS1 silence were measured by western blot (WB). g-j The mRNA levels of AXIN2, LGR5, SOX4 and MYC were positively correlated with the expression of SLCO4A1-AS1 in the 50 CRC samples. qRT-PCR was performed and GAPDH acted as loading control. *p < 0.05 and **p < 0.01