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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Vitexin compound 1, a novel extraction from a Chinese herb, suppresses melanoma cell growth through DNA damage by increasing ROS levels

Fig. 6

VB1 induces DNA damage by increasing ROS in RA cells. a RA cells were treated with 0–20 μM VB1 for 48 h, and western blotting was then performed for the indicated antibodies. b & c RA cells were treated with 10 μM VB1 for 0–48 h, and γH2AX was stained by immunofluorescence (c), the foci of γH2AX was quantificated (b), the results represent the mean (n = 5) ± SD of each group, and an asterisk (*) indicates a significant difference using one-way ANOVA (p < 0.05). d RA cells were treated with 20 μM VB1 for 0–12 h. The levels of ROS were measured by DCF fluorescence with flow cytometry. The relative ROS levels were analyzed by GraphPad Prism software (histogram) (left panel). RA cells were pretreated with 5 mmol/L N-acetylcysteine (NAC) for 1 h, and then exposed to 20 μM VB1 for another 6 h. The levels of ROS were measured by DCF fluorescence with flow cytometry. The relative ROS levels were analyzed using the GraphPad Prism software (histogram) (right panel). The results represent the mean (n = 4) ± SD of each group, and an asterisk (*) indicates a significant difference using one-way ANOVA (p < 0.05)

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